Nováková Lucie, Solichová Dagmar, Pavlovicová Sona, Solich Petr
Department of Metabolic Care and Gerontology, Charles University, Faculty of Medicine and University Hospital in Hradec Králové, Hradec Králové, Czech Republic.
J Sep Sci. 2008 May;31(9):1634-44. doi: 10.1002/jssc.200700570.
Hydrophilic interaction liquid chromatography (HILIC) method using internal standard for the determination and stability study of ascorbic acid was developed. HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150 x 2.1 mm, 3.5 microm) chromatographic column and mobile phase composed of ACN and 50 mM ammonium acetate buffer pH 6.8 (78:22 v/v). Diode array detection was performed and chromatograms were processed at 268 nm, the maximum wavelength of absorbance of ascorbic acid. An extensive stability study of ascorbic acid as a function of various factors including temperature, stabilizing agents, oxygen presence and its concentration in solution was performed in order to gain information about the quantitative influence of individual stability factors. Low temperature and stabilizing agents (o-phosphoric acid and oxalic acid) were found to be key factors enabling substantial enhancement of the stability of ascorbic acid.
建立了一种采用内标法的亲水作用液相色谱（HILIC）法用于测定抗坏血酸及其稳定性研究。在以下分析条件下，HILIC法快速且简便：使用ZIC HILIC（150×2.1 mm，3.5微米）色谱柱，流动相由乙腈和pH 6.8的50 mM醋酸铵缓冲液（78:22 v/v）组成。采用二极管阵列检测，在268 nm（抗坏血酸吸光度的最大波长）处处理色谱图。对抗坏血酸在包括温度、稳定剂、氧气存在及其在溶液中的浓度等各种因素作用下的稳定性进行了广泛研究，以获取有关各个稳定性因素定量影响的信息。发现低温和稳定剂（邻磷酸和草酸）是能够显著提高抗坏血酸稳定性的关键因素。
Zotero 插件