采用亲水作用色谱-紫外检测法对手洗餐具用洗涤剂中乳酸的定量分析。

Lactic acid quantitation in hand dishwashing liquid using an HILIC-UV methodology.

机构信息

Global Analytical Science Department, Colgate-Palmolive Company, Piscataway, NJ 08855, USA.

出版信息

J Sep Sci. 2010 Mar;33(6-7):982-7. doi: 10.1002/jssc.200900697.

DOI:10.1002/jssc.200900697

PMID:20155748

Abstract

Different hydrophilic interaction chromatography (HILIC) columns were screened for lactic acid separation in hand dishwashing liquid products and the influence of mobile phase strength, buffer concentration and column temperature on the retention of lactic acid on a Zorbax NH(2) column was investigated. An isocratic HILIC method for the quantitation of lactic acid in hand dishwashing liquid products was developed. The mobile phase consists of 70% methanol and 30% 20 mM sodium phosphate buffer (v/v) at pH 2.5. The HILIC stationary phase is Zorbax NH(2), 250x4.6 with a 5 microm particle size. Detection was carried out using a variable wavelength UV-VIS detector at 226 nm. The linear range and percent recovery for lactic acid in the products were 44.68-1206.39 microg/mL and 100.3%, respectively. This paper provides an optimized HILIC methodology for the analysis of an acidic polar analyte (lactic acid) on a basic stationary phase. The proposed method can be used for the routine analysis of lactic acid.

摘要

不同的亲水相互作用色谱（HILIC）柱被筛选用于手洗餐具产品中的乳酸分离，并研究了流动相强度、缓冲液浓度和柱温对 Zorbax NH（2）柱上乳酸保留的影响。开发了一种用于手洗餐具产品中乳酸定量的等度 HILIC 方法。流动相由 70%甲醇和 30%20mM 磷酸钠缓冲液（v/v）组成，pH 值为 2.5。HILIC 固定相为 Zorbax NH（2），250x4.6，粒径为 5 微米。检测采用可变波长紫外-可见检测器在 226nm 处进行。产品中乳酸的线性范围和回收率分别为 44.68-1206.39μg/mL 和 100.3%。本文提供了一种优化的 HILIC 方法，用于在碱性固定相上分析酸性极性分析物（乳酸）。该方法可用于乳酸的常规分析。