利用吸收光谱和荧光光谱对DNA和RNA进行定量分析。
Quantitation of DNA and RNA with absorption and fluorescence spectroscopy.
作者信息
Gallagher S R
机构信息
Motorola Corporation, Tempe, Arizona, USA.
出版信息
Curr Protoc Neurosci. 2001 May;Appendix 1:Appendix 1K. doi: 10.1002/0471142301.nsa01ks10.
PMID:18428442
Abstract
Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques are presented below. These three procedures cover a range from 5 to 10 ng/ml DNA to 50 mg/ml DNA.
摘要
对溶液中纳克和微克量的DNA和RNA进行可靠定量，对分子生物学领域的研究人员至关重要。除了传统的260nm吸光度测量方法外，下面还介绍了另外两种更灵敏的荧光技术。这三种方法涵盖了从5至10 ng/ml DNA到50 mg/ml DNA的范围。
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