用于亚磷酰胺 RNA 合成的嘌呤核糖核苷的区域选择性 2'-硅烷化反应
Regioselective 2'-silylation of purine ribonucleosides for phosphoramidite RNA synthesis.
作者信息
Gaffney B L, Jones R A
机构信息
Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
出版信息
Curr Protoc Nucleic Acid Chem. 2001 Nov;Chapter 2:Unit 2.8. doi: 10.1002/0471142700.nc0208s06.
This unit describes high-yield procedures for protection of purine ribonucleosides based on a reaction that allows concomitant highly regioselective 2'-silylation and 3'-phosphitylation to give, in one step, monomers that are ready for H-phosphonate synthesis. For preparation of phosphoramidites, the H-phosphonate monoester is cleaved without silyl migration to give intermediates ready for phosphitylation by standard methods.
本单元描述了基于一种反应的嘌呤核糖核苷保护的高产率方法，该反应允许同时进行高度区域选择性的2'-硅烷化和3'-亚磷酸酯化，一步得到可用于H-亚磷酸酯合成的单体。对于亚磷酰胺的制备，H-亚磷酸酯单酯被裂解而不发生硅烷基迁移，得到可通过标准方法进行亚磷酸酯化的中间体。
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