Solid-phase synthesis of 2'-deoxy-2'-fluoro- beta-D-oligoarabinonucleotides (2'F-ANA) and their phosphorothioate derivatives.
作者信息
Viazovkina Ekaterina, Mangos Maria M, Elzagheid Mohamed I, Damha Masad J
机构信息
McGill University, Montreal, Canada.
出版信息
Curr Protoc Nucleic Acid Chem. 2002 Nov;Chapter 4:Unit 4.15. doi: 10.1002/0471142700.nc0415s10.
PMID:18428897
Abstract
This unit describes the chemical synthesis of 2'-deoxy-2'-fluoro-b-D-oligoarabinonucleotides (2'F-ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite preparation, assembly on DNA synthesizers, and final deprotection and purification of oligonucleotides.
摘要
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