Mikhailov Sergey N, Efimtseva Ekaterina V, Rodionov Andrei A, Bobkov Georgii V, Kulikova Irina V, Herdewijn Piet
Russian Academy of Sciences, Engelhardt Institute of Molecular Biology, Moscow, Russia.
Curr Protoc Nucleic Acid Chem. 2007 Jan;Chapter 1:Unit 1.14. doi: 10.1002/0471142700.nc0114s27.
A simple and efficient method for the preparation of 2-O-beta-D-ribofuranosylnucleosides, minor tRNA components, is described in this unit. The method consists of condensation of a small excess of 1-O-acetyl-2,3,5-tri-O-benzoyl-beta-D-ribofuranose activated with tin tetrachloride with N-protected 3,5-O-tetra-isopropyldisiloxane-1,3-diyl-ribonucleosides in 1,2-dichloroethane. Subsequent deprotection produces 2-O-beta-D-ribofuranosylnucleosides in an overall yield of 46% to 72%.
本单元介绍了一种制备2-O-β-D-呋喃核糖核苷（微量tRNA成分）的简单有效方法。该方法包括在1,2-二氯乙烷中，用四氯化锡活化的少量过量1-O-乙酰基-2,3,5-三-O-苯甲酰基-β-D-呋喃核糖与N-保护的3,5-O-四异丙基二硅氧烷-1,3-二基核糖核苷缩合。随后的脱保护反应生成2-O-β-D-呋喃核糖核苷，总产率为46%至72%。
