使用硫酸铵进行蛋白质沉淀。
Protein precipitation using ammonium sulfate.
作者信息
Wingfield P
机构信息
National Institutes of Health, Bethesda, Maryland, USA.
出版信息
Curr Protoc Protein Sci. 2001 May;Appendix 3:Appendix 3F. doi: 10.1002/0471140864.psa03fs13.
Abstract
The basic theory of protein precipitation by addition of ammonium sulfate is presented and the most common applications are listed. Numerous tables are provided for calculating the appropriate amount of ammonium sulfate to add to a particular protein solution.
摘要
介绍了通过添加硫酸铵沉淀蛋白质的基本理论，并列出了最常见的应用。提供了许多表格，用于计算向特定蛋白质溶液中添加硫酸铵的合适量。
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Tables for the preparation of ammonium sulfate solutions.硫酸铵溶液配制表。
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