Righetti P G, Bossi A, Gelfi C
University of Verona, Verona, Italy.
Curr Protoc Protein Sci. 2001 May;Chapter 10:Unit 10.13. doi: 10.1002/0471140864.ps1013s16.
Capillary electrophoresis in acidic isoelectric buffers is a novel methodology allowing fast protein and peptide analysis in uncoated capillaries. Due to the low pH adopted and to the use of dynamic coating with cellulose derivatives, silanol ionization is essentially suppressed and no interaction of macromolecules with the untreated wall ensues. In addition, because of the low conductivity of quasi-stationary isoelectric buffers, high voltage gradients can be applied (up to 800 V/cm), permitting fast peptide analysis with a high resolving power and minimal diffusional peak spreading.
在酸性等电缓冲液中进行毛细管电泳是一种新颖的方法，可在未涂层的毛细管中快速分析蛋白质和肽。由于采用了低pH值并使用了纤维素衍生物进行动态涂层，硅醇的电离基本上受到抑制，大分子不会与未处理的管壁发生相互作用。此外，由于准静态等电缓冲液的低电导率，可以施加高电压梯度（高达800 V/cm），从而能够以高分辨率和最小的扩散峰展宽快速分析肽。
