Henzel W J, Stults J T
Genentech, Inc., South San Francisco, California, USA.
Curr Protoc Protein Sci. 2001 May;Chapter 16:Unit 16.2. doi: 10.1002/0471140864.ps1602s04.
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is one of the most useful techniques for determining the mass of biomolecules, with exceptional capabilities for mass analysis of peptides. Relative to other ionization techniques, it provides high sensitivity and excellent tolerance of salt and other common buffer components. Routine detection limits for peptides are in the subpicomole range. The ions commonly observed are the protonated molecules (M+H(+)), which makes data analysis relatively easy. This overview discusses instrument configuration and calibration, sample preparation, along with specific approaches for analyzing peptide mixtures, synthetic peptides, and chemical modifications of peptides.
基质辅助激光解吸/电离质谱（MALDI-MS）是测定生物分子质量最有用的技术之一，在肽的质量分析方面具有卓越的能力。相对于其他电离技术，它具有高灵敏度以及对盐和其他常见缓冲液成分的出色耐受性。肽的常规检测限在亚皮摩尔范围内。通常观察到的离子是质子化分子（M+H(+)），这使得数据分析相对容易。本综述讨论了仪器配置与校准、样品制备，以及分析肽混合物、合成肽和肽的化学修饰的具体方法。
