Chen Lin, Annis Ioana, Barany George
AxCell Biosciences Corporation, Newtown, Pennsylvania.
Union Carbide Corporation, Bound Brook, New Jersey.
Curr Protoc Protein Sci. 2001 May;Chapter 18:18.6.1-18.6.19. doi: 10.1002/0471140864.ps1806s23.
The formation of disulfide bridges is often a crucial final stage in peptide synthesis. There is compelling evidence that the disulfide pattern can be critical in the folding and structural stabilization of many natural peptide and protein sequences, while the artificial introduction of disulfide bridges into natural or designed peptides may often improve biological activities/specificities and stabilities. This unit provides a highly selective, albeit state-of-the-art, menu of procedures that can be performed to establish intramolecular or intermolecular disulfide bridges in targets of varying complexities.
二硫键的形成通常是肽合成中至关重要的最后阶段。有令人信服的证据表明，二硫键模式在许多天然肽和蛋白质序列的折叠和结构稳定中可能至关重要，而将二硫键人工引入天然或设计的肽中往往可以提高生物活性/特异性和稳定性。本单元提供了一系列高度选择性的、尽管是最先进的程序，可用于在不同复杂程度的目标中建立分子内或分子间二硫键。
