使用BLAST系列程序进行序列相似性搜索。
Sequence similarity searching using the BLAST family of programs.
作者信息
Wolfsberg T G, Madden T L
机构信息
National Center for Biotechnology Information, National Library of Medicine, NIH, Bethesda, Maryland, USA.
出版信息
Curr Protoc Protein Sci. 2001 May;Chapter 2:Unit2.5. doi: 10.1002/0471140864.ps0205s15.
The BLAST (Basic Local Alignment Search Tool) family of sequence similarity search programs allows users to input either a nucleotide or amino acid query sequence, and search a nucleotide or amino acid sequence database. The program returns a list of the sequence "hits", alignments to the query sequence, and statistical values. This unit describes how to choose an appropriate BLAST program and database, perform the search, and interpret the results.
BLAST（基本局部比对搜索工具）系列序列相似性搜索程序允许用户输入核苷酸或氨基酸查询序列，并搜索核苷酸或氨基酸序列数据库。该程序返回序列“命中结果”列表、与查询序列的比对以及统计值。本单元介绍如何选择合适的BLAST程序和数据库、进行搜索以及解释结果。
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