Allison R D
University of Florida, Gainesville, Florida, USA.
Curr Protoc Protein Sci. 2001 May;Chapter 3:Unit 3.5. doi: 10.1002/0471140864.ps0305s05.
The purpose of this unit is to provide a brief review of issues important in the design of initial-rate assay methods. General aspects of kinetic assay design are discussed, including enzyme and substrate purity, concentration and stability. Also covered are issues such as continuous versus stop-time assay formats, coupled enzyme assays, binding studies, and presentation of initial-rate data.
本单元的目的是简要回顾初速率测定方法设计中重要的问题。讨论了动力学测定设计的一般方面，包括酶和底物的纯度、浓度及稳定性。还涵盖了诸如连续测定与终止时间测定形式、偶联酶测定、结合研究以及初速率数据呈现等问题。
