Link Andrew J, Jennings Jennifer L, Washburn Michael P
Vanderbilt University School of Medicine, Nashville, Tennessee.
Torrey Mesa Research Institute, San Diego, California.
Curr Protoc Protein Sci. 2004 Feb;Chapter 23:23.1.1-23.1.25. doi: 10.1002/0471140864.ps2301s34.
Multidimensional liquid chromatography of peptides produced by protease digestion of complex protein mixtures followed by tandem mass spectrometry can be coupled with automated database searching to identify large numbers of proteins in complex samples. These methods avoid the limitations of gel electrophoresis and in-gel digestions by directly identifying protein mixtures in solution.
对复杂蛋白质混合物进行蛋白酶消化后产生的肽段进行多维液相色谱，随后进行串联质谱分析，可与自动数据库搜索相结合，以鉴定复杂样品中的大量蛋白质。这些方法通过直接鉴定溶液中的蛋白质混合物，避免了凝胶电泳和胶内消化的局限性。
