一种用于评估噬菌体对铜绿假单胞菌生物膜影响的比色微量滴定板法。

A colorimetric microtiter plate method for assessment of phage effect on Pseudomonas aeruginosa biofilm.

作者信息

Knezevic Petar, Petrovic Olga

机构信息

Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21 000 Novi Sad, Vojvodina, Serbia.

出版信息

J Microbiol Methods. 2008 Aug;74(2-3):114-8. doi: 10.1016/j.mimet.2008.03.005. Epub 2008 Mar 14.

DOI:10.1016/j.mimet.2008.03.005

PMID:18433900

Abstract

Bacteriophages have a potential in biofilm control. The aim of the study was to develop a method for selection of the most effective Pseudomonas aeruginosa phages for inhibition of biofilm formation and its eradication. The microtiter plate method is based on crystal violet staining and measuring of optical density.

摘要

噬菌体在生物膜控制方面具有潜力。本研究的目的是开发一种方法，用于筛选最有效的铜绿假单胞菌噬菌体，以抑制生物膜形成并将其根除。微量滴定板法基于结晶紫染色和光密度测量。