Trapali M, Liapi C, Perelas A, Perrea D, Stroubini T, Dontas I, Couvari E, Mavri M, Galanopoulou P
Department of Pharmacology, Medical School, University of Athens, Athens, Greece.
Pharmacology. 2008;82(1):15-21. doi: 10.1159/000127363. Epub 2008 Apr 22.
BACKGROUND/AIM: The effect of isocaloric diets and sibutramine on dietary behaviour and TNF-alpha is poorly understood. The aim of the study was to investigate the effects of isocaloric diets and sibutramine on food intake, body mass variation and serum TNF-alpha in free-feeding rats.
Three groups of male Wistar rats (n = 63) were fed a high-fat diet, high-carbohydrate diet or high-protein diet for 13 weeks. In the last 3 weeks, each group was divided into 3 subgroups. Each subgroup received sibutramine 5 mg/kg, sibutramine 10 mg/kg or vehicle. Food intake was measured daily during the last week of the experiment; serum TNF-alpha was assayed and the body weight increasing rate was calculated.
The high-fat diet was associated with increased food intake, a greater weight gain ratio and increased TNF-alpha levels. Sibutramine treatment did not affect the dietary behaviour of high-protein- or high-carbohydrate-fed rats, while it significantly attenuated the daily food intake and body weight gain rate in the high-fat group, at the dose of 10 mg/kg. TNF-alpha levels were not affected by sibutramine.
High-fat feeding was associated with an increase in daily food intake, TNF-alpha levels and body weight gain rate, as well as with enhanced responsiveness to the anorectic effects of sibutramine. However, sibutramine did not affect TNF-alpha.
背景/目的：等热量饮食和西布曲明对饮食行为及肿瘤坏死因子-α（TNF-α）的影响尚不清楚。本研究旨在探讨等热量饮食和西布曲明对自由进食大鼠食物摄入量、体重变化及血清TNF-α的影响。
将三组雄性Wistar大鼠（n = 63）分别给予高脂饮食、高碳水化合物饮食或高蛋白饮食13周。在最后3周，每组再分为3个亚组。每个亚组分别给予5 mg/kg西布曲明、10 mg/kg西布曲明或赋形剂。在实验的最后一周每天测量食物摄入量；检测血清TNF-α并计算体重增加率。
高脂饮食与食物摄入量增加、体重增加比例更高及TNF-α水平升高有关。西布曲明治疗对高蛋白或高碳水化合物喂养的大鼠的饮食行为没有影响，而在10 mg/kg剂量时，它显著降低了高脂组的每日食物摄入量和体重增加率。西布曲明对TNF-α水平没有影响。
高脂喂养与每日食物摄入量增加、TNF-α水平及体重增加率升高有关，也与对西布曲明的厌食作用反应增强有关。然而，西布曲明不影响TNF-α。
Zotero 插件