Stroubini Th, Perelas A, Liapi C, Perrea D, Dontas I, Tzavara Ch, Galanopoulou P
Department of Pharmacology, Medical School, University of Athens, 75, M. Asias street, Goudi 11527, Athens, Greece.
Cytokine. 2009 May;46(2):171-5. doi: 10.1016/j.cyto.2009.01.003. Epub 2009 Feb 28.
The aim of the study was to investigate the effects of (1) macronutrients on food intake, body composition and serum resistin and adiponectin and (2) sibutramine(S) on the above parameters in rats fed with isocaloric diets. Three groups of male Wistar rats (n=63) were fed with high fat diet (HFD), high carbohydrate diet (HCD) or high protein diet (HPD) for 13weeks. In the last 3weeks each group was divided into three subgroups and received S 5mg/kg or 10mg/kg, or vehicle. Body weight was measured weekly, gastrocnemius muscle, perirenal, retroperitoneal and epididymal fat were isolated, fat/lean ratio was calculated and serum adiponectin and resistin were assayed. S did not affect lean body mass in any group. HFD was associated with elevated fat/lean ratio regardless of S administration. S at 10mg/Kg decreased fat/lean ratio in the HCD and HPD and adiponectin in the HFD group. S did not affect resistin in any group. Adiponectin was paradoxically elevated in the HFDS10 compared to the HCD or HPD S10 groups. Resistin was lower in the HCD compared to the HPD and HFD groups. Results suggest a preferential effect of S on body fat. The detrimental effect of S on adiponectin can be attributed to its sympathomimetic properties. Adiponectin was paradoxically elevated in the HFD and resistin in the HPD group, results that require further investigation.
(1) 常量营养素对食物摄入量、身体成分、血清抵抗素和脂联素的影响；(2) 西布曲明（S）对喂食等热量饮食的大鼠上述参数的影响。将三组雄性Wistar大鼠（n = 63）分别喂食高脂饮食（HFD）、高碳水化合物饮食（HCD）或高蛋白饮食（HPD）13周。在最后3周，每组再分为三个亚组，分别给予5mg/kg或10mg/kg的S或赋形剂。每周测量体重，分离腓肠肌、肾周、腹膜后和附睾脂肪，计算脂肪/瘦肉比率，并测定血清脂联素和抵抗素。S对任何组的去脂体重均无影响。无论是否给予S，HFD均与脂肪/瘦肉比率升高有关。10mg/Kg的S可降低HCD和HPD组的脂肪/瘦肉比率以及HFD组的脂联素。S对任何组的抵抗素均无影响。与HCD或HPD S10组相比，HFDS10组的脂联素反而升高。与HPD和HFD组相比，HCD组的抵抗素较低。结果表明S对体脂有优先作用。S对脂联素的有害作用可归因于其拟交感神经特性。HFD组的脂联素反而升高，HPD组的抵抗素升高，这些结果需要进一步研究。
