Peng Baowei, Chu Maoquan
College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008 Feb;25(1):72-6.
Quantum dots (QDs) have favorable physical and photochemical properties. In this work, we used QDs fluorescent nanoprobes to follow the migration of inflammatory cells from local tissue to draining lymph node in inflammation resolution. Electric pulse stimulation was used to establish inflammation model in mouse tibialis anterior. QDs injected to inflammatory tissue were found to aggregate and endocytosed by inflammatory cells. While in the draining lymph node, QDs mainly distributed in the T cell area. TEM and confocal observation showed that most of QDs in the draining lymph node were located in the endosomes of monocytes/macrophages. Our work shows that QDs can be used as fluorescent probes to follow migration of cells especially phagocytic cells. Thus QDs may be explored in application in immunology research.
量子点（QDs）具有良好的物理和光化学性质。在本研究中，我们使用量子点荧光纳米探针追踪炎症消退过程中炎症细胞从局部组织向引流淋巴结的迁移。采用电脉冲刺激建立小鼠胫前肌炎症模型。发现注入炎症组织的量子点会聚集并被炎症细胞内吞。而在引流淋巴结中，量子点主要分布在T细胞区。透射电子显微镜（TEM）和共聚焦观察表明，引流淋巴结中的大多数量子点位于单核细胞/巨噬细胞的内体中。我们的研究表明，量子点可作为荧光探针追踪细胞尤其是吞噬细胞的迁移。因此，量子点在免疫学研究中的应用值得探索。
