Maysinger Dusica, Lovrić Jasmina, Eisenberg Adi, Savić Radoslav
Department of Pharmacology and Therapeutics, McGill University, Montreal, Canada.
Eur J Pharm Biopharm. 2007 Mar;65(3):270-81. doi: 10.1016/j.ejpb.2006.08.011. Epub 2006 Sep 1.
Micelles and quantum dots have been used as experimental drug delivery systems and imaging tools both in vitro and in vivo. Investigations of their fate at the subcellular level require different surface-core modifications. Among the most common modifications are those with fluorescent probes, dense-core metals or radionucleids. Cellular fate of several fluorescent probes incorporated into poly(caprolactone)-b-copolymer micelles (PCL-b-PEO) was followed by confocal microscopy, and colloidal gold incorporated in poly 4-vinyl pyridine-PEO micelles were developed to explore micelle fate by electron microscopy. More recently, we have examined quantum dots (QDs) as the next-generation-labels for cells and nanoparticulate drug carriers amenable both to confocal and electron microscopic analyses. Effects of QDs at the cellular and subcellular levels and their integrity were studied. Results from different studies suggest that size, charge and surface manipulations of QDs may play a role in their subcellular distribution. Examples of pharmacological agents incorporated into block copolymer micelles, administered or attached to QD surfaces show how the final biological outcome (e.g. cell death, proliferation or differentiation) depends on physical properties of these nanoparticles.
胶束和量子点已被用作体外和体内实验性药物递送系统及成像工具。在亚细胞水平研究它们的命运需要进行不同的表面-核心修饰。最常见的修饰包括用荧光探针、致密核心金属或放射性核素进行修饰。通过共聚焦显微镜追踪了几种掺入聚（己内酯）-b-共聚物胶束（PCL-b-PEO）中的荧光探针的细胞命运，并且开发了掺入聚4-乙烯基吡啶-PEO胶束中的胶体金，以通过电子显微镜探索胶束命运。最近，我们研究了量子点（QDs）作为细胞的下一代标记物以及适用于共聚焦和电子显微镜分析的纳米颗粒药物载体。研究了量子点在细胞和亚细胞水平的作用及其完整性。不同研究的结果表明，量子点的大小、电荷和表面操作可能在其亚细胞分布中起作用。掺入嵌段共聚物胶束、给药或附着在量子点表面的药物制剂实例表明，最终的生物学结果（例如细胞死亡、增殖或分化）如何取决于这些纳米颗粒的物理性质。
