Fatar M, Stroick M, Griebe M, Alonso A, Kreisel S, Kern R, Hennerici M, Meairs S
Department of Neurology, Universitätsklinikum Mannheim, University of Heidelberg, Heidelberg, Germany.
Ultrasound Med Biol. 2008 Sep;34(9):1414-20. doi: 10.1016/j.ultrasmedbio.2008.02.008. Epub 2008 Apr 24.
Combined 2-MHz ultrasound (US) and second-generation, sulfur hexafluoride microbubbles (MB) treatment (US+MB) was performed in a permanent middle cerebral artery (MCA) occlusion model in rats to evaluate possible effects on the ischemic cascade. We used 16 Wistar rats and the MCA occlusion model for stroke induction. Glutamate, pyruvate, lactate and glycerol levels were measured by intracerebral microdialysis before and after stroke induction and after US+MB application (n = 8) for 20 h. After 24 h, brain infarct volume, apoptosis and IL-6 and TNF-alpha levels were evaluated. The infarct volume was significantly reduced (p < 0.05) in the US+MB-treated group compared with control animals. In additional, glutamate levels were significantly lower in US+MB-treated animals, and these animals showed a higher rate of apoptotic cell death in the infarcted area. The levels of IL-6 and TNF-alpha concentrations were not different in both groups, and there was no apoptotic cell death outside the infarction in animals treated with US+MB. The results demonstrate that US+MB with second generation microbubbles does not have a harmful effect on ischemic stroke in an MCA occlusion model of the rat.
在大鼠永久性大脑中动脉（MCA）闭塞模型中进行2兆赫超声（US）与第二代六氟化硫微泡（MB）联合治疗（US+MB），以评估其对缺血级联反应的可能影响。我们使用16只Wistar大鼠和MCA闭塞模型来诱导中风。在中风诱导前后以及US+MB应用20小时后（n = 8），通过脑微透析测量谷氨酸、丙酮酸、乳酸和甘油水平。24小时后，评估脑梗死体积、细胞凋亡以及白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平。与对照动物相比，US+MB治疗组的梗死体积显著减小（p < 0.05）。此外，US+MB治疗的动物中谷氨酸水平显著降低，并且这些动物在梗死区域显示出更高的凋亡细胞死亡率。两组中IL-6和TNF-α浓度水平没有差异，并且在接受US+MB治疗的动物中梗死灶外没有凋亡细胞死亡。结果表明，在大鼠MCA闭塞模型中，第二代微泡的US+MB对缺血性中风没有有害影响。
