Jaklič Domen, Lapanje Aleš, Zupančič Klemen, Smrke Dragica, Gunde-Cimerman Nina
Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000 Ljubljana, Slovenia.
Institute of Physical Biology, Veliko Mlačevo 59, 1290 Grosuplje, Slovenia.
J Med Microbiol. 2008 May;57(Pt 5):617-625. doi: 10.1099/jmm.0.47515-0.
Maggot therapy, also known as biosurgery, is an ancient method for the healing of chronic infected wounds. Although clinicians have reported on the beneficial activities of the Lucilia sericata larvae that have been used for healing chronic wounds, the selectivity of this therapy against the different pathogenic micro-organisms that are found in chronic wounds has never been analysed. In the present study, we have investigated the in vitro activities of larval excreta/secreta both against selected bacterial strains that frequently occur in chronically infected wounds, and against bacteria isolated directly from the larvae and their excreta/secreta. Additionally, the antibacterial activities were investigated in in vivo studies, by comparing bacterial diversity in wounds before and after the application of L. sericata larvae. In conclusion, larval therapy is highly recommended, particularly for the treatment of wounds infected with Gram-positive bacteria, like Staphylococcus aureus, but less so for wounds infected with Gram-negative bacteria, especially Proteus spp. and Pseudomonas spp. strains. Bacteria from the genus Vagococcus were resistant to the maggot excreta/secreta.
蛆虫疗法，也称为生物外科手术，是一种治疗慢性感染伤口的古老方法。尽管临床医生已经报道了用于治疗慢性伤口的丝光绿蝇幼虫的有益作用，但这种疗法对慢性伤口中发现的不同致病微生物的选择性从未得到分析。在本研究中，我们研究了幼虫排泄物/分泌物对慢性感染伤口中常见的选定细菌菌株以及直接从幼虫及其排泄物/分泌物中分离出的细菌的体外活性。此外，通过比较应用丝光绿蝇幼虫前后伤口中的细菌多样性，在体内研究中调查了抗菌活性。总之，强烈推荐幼虫疗法，特别是用于治疗感染革兰氏阳性菌（如金黄色葡萄球菌）的伤口，但对于感染革兰氏阴性菌（尤其是变形杆菌属和假单胞菌属菌株）的伤口则不太推荐。漫游球菌属的细菌对蛆虫排泄物/分泌物具有抗性。
