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来自家鸡（Gallus gallus）的全长禽白血病病毒相关内源性逆转录病毒ChiRV1的分子特征分析

Molecular characterization of full-length MLV-related endogenous retrovirus ChiRV1 from the chicken, Gallus gallus.

作者信息

Borysenko Leonid, Stepanets Volodymir, Rynditch Alla V

机构信息

Department of Functional Genomics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo street, 03143 Kiev, Ukraine.

出版信息

Virology. 2008 Jun 20;376(1):199-204. doi: 10.1016/j.virol.2008.03.006. Epub 2008 Apr 25.

DOI:10.1016/j.virol.2008.03.006

PMID:18440041

Abstract

We report the first full-length sequence of an endogenous retrovirus from the genome of domestic chicken, that is not related to the Avian leukemia viruses (ALV). This retrovirus, designated ChiRV1, clusters with Murine leukemia virus (MLV)-related retroviruses and hence is the first complete gammaretrovirus from the genome of a bird. Nevertheless it is not related to exogenous MLV-related retroviruses infecting chicken. The provirus is 9133 bp long and contains 90%-identical LTRs as well as reading frames for the gag, pol and env genes, interrupted by in-frame stop codons. Expression analysis showed that ChiRV1 is a transcribed provirus. Screening of the chicken genome database revealed 100 ChiRV1-related sequences that are grouped into three classes based upon LTR alignment and subsequent phylogenetic analysis.

摘要

我们报道了首个来自家鸡基因组的内源性逆转录病毒的全长序列，该病毒与禽白血病病毒（ALV）无关。这种逆转录病毒被命名为ChiRV1，与鼠白血病病毒（MLV）相关的逆转录病毒聚类，因此是来自鸟类基因组的首个完整的γ逆转录病毒。然而，它与感染鸡的外源性MLV相关逆转录病毒无关。前病毒长9133 bp，包含90%相同的长末端重复序列（LTR）以及gag、pol和env基因的阅读框，被框内终止密码子打断。表达分析表明ChiRV1是一个转录的前病毒。对鸡基因组数据库的筛选揭示了100个与ChiRV1相关的序列，根据LTR比对和随后的系统发育分析，这些序列被分为三类。