Hsieh Shih-Chuan, Fang Shih-Hua, Rao Yerra Koteswara, Tzeng Yew-Min
Institute of Biotechnology, Chaoyang University of Technology, Wufeng, Taiwan, ROC.
J Ethnopharmacol. 2008 Jun 19;118(1):65-70. doi: 10.1016/j.jep.2008.03.003. Epub 2008 Mar 14.
Anisomeles indica (L.) Kuntze (Labiatae), is a traditional anti-inflammatory herb used in Taiwan. The aqueous and methanolic extracts of whole plants, leaves, flowers and stems; and chloroform and n-butanol fractions of methanol extract, from A. indica were investigated for their anti-inflammatory activity on murine peritoneal macrophages. In addition, the tumor cells proliferation inhibition activities of these extracts were also evaluated against a panel of tumor cell lines such as Colon 205, PC 3, HepG2 and MCF 7. Treatment with A. indica extracts did not reduce cell viability at any dose used. However, all the extracts significantly inhibited the enhanced production of NO radicals, and pro-inflammatory cytokines (TNF-alpha, and IL-12) induced by LPS/IFN-gamma in a dose-dependent manner. Furthermore, methanolic extracts of leaves and flowers significantly and dose-dependently arrest mitogen-stimulated spleen cells in G0/G1 stage, in addition to their cell proliferation inhibition against Colon 205, MCF 7 and PC 3 by 94, 82; 98, 71; 82, 98%, respectively, at 200 microg/mL concentration. This is the first report on A. indica extracts for their growth inhibitory activities, against inflammatory mediator production, and human tumor cell lines, colon, prostate, hepatoma and breast cells proliferation.
印度罗勒（Anisomeles indica (L.) Kuntze，唇形科）是台湾一种传统的抗炎草药。对印度罗勒全株、叶、花和茎的水提取物和甲醇提取物，以及甲醇提取物的氯仿和正丁醇馏分，进行了其对小鼠腹腔巨噬细胞抗炎活性的研究。此外，还针对一组肿瘤细胞系，如结肠205、PC 3、HepG2和MCF 7，评估了这些提取物对肿瘤细胞增殖的抑制活性。在所用的任何剂量下，用印度罗勒提取物处理均未降低细胞活力。然而，所有提取物均以剂量依赖的方式显著抑制了由脂多糖/γ干扰素诱导的一氧化氮自由基和促炎细胞因子（肿瘤坏死因子-α和白细胞介素-12）的过量产生。此外，叶和花的甲醇提取物除了在200μg/mL浓度下对结肠205、MCF 7和PC 3的细胞增殖抑制率分别为94%、82%；98%、71%；82%、98%外，还能显著且剂量依赖性地使丝裂原刺激的脾细胞停滞在G0/G1期。这是关于印度罗勒提取物对炎症介质产生的抑制活性以及对人肿瘤细胞系（结肠、前列腺、肝癌和乳腺癌细胞）增殖的生长抑制活性的首次报道。
