通过向反硝化假单胞菌投喂甜菜碱提高维生素B12的大规模生产。
Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding.
作者信息
Li Kun-Tai, Liu Dong-Hong, Li Yong-Liang, Chu Ju, Wang Yong-Hong, Zhuang Ying-Ping, Zhang Si-Liang
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; College of Biological Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
出版信息
Bioresour Technol. 2008 Nov;99(17):8516-20. doi: 10.1016/j.biortech.2008.03.023. Epub 2008 Apr 25.
The strategy of betaine control for vitamin B12 large-scale fermentation by Pseudomonas denitrificans was investigated in this paper. The results obtained in shake-flask experiments demonstrated that betaine could greatly stimulate vitamin B12 biosynthesis but had an inhibition to cell growth. Based on the influence of betaine on the fermentation of P. denitrificans, betaine feeding was a beneficial strategy to solve the inconsistency between cell growth and vitamin B12 production. As a result, an effective and economical strategy of betaine feeding was established for vitamin B12 fermentation in 120-m3 fermenter, in which betaine was continuously fed to maintain betaine concentration of the broth at the range of 5-7g/l during 50-140h of fermentation.
本文研究了反硝化假单胞菌大规模发酵生产维生素B12时甜菜碱的控制策略。摇瓶实验结果表明，甜菜碱能极大地刺激维生素B12的生物合成，但对细胞生长有抑制作用。基于甜菜碱对反硝化假单胞菌发酵的影响，甜菜碱补料是解决细胞生长与维生素B12生产不一致问题的有益策略。因此，建立了一种有效且经济的甜菜碱补料策略用于120立方米发酵罐中维生素B12的发酵，即在发酵50 - 140小时期间持续补加甜菜碱，使发酵液中甜菜碱浓度维持在5 - 7克/升。
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