引起西班牙阿斯图里亚斯地区两次局部暴发的两种腮腺炎病毒基因型的分子鉴定
Molecular identification of two genotypes of mumps virus causing two regional outbreaks in Asturias, Spain.
作者信息
Boga J A, de Oña M, Fernández-Verdugo A, González D, Morilla A, Arias M, Barreiro L, Hidalgo F, Melón S
机构信息
Servicio de Microbiología, Hospital Universitario Central de Asturias, Oviedo, Spain.
出版信息
J Clin Virol. 2008 Aug;42(4):425-8. doi: 10.1016/j.jcv.2008.03.010. Epub 2008 Apr 28.
BACKGROUND
In spite of universal vaccination, several sporadic cases of mumps infection, which could produce outbreaks, are detected every year in different countries.
OBJECTIVE
Mumps virus strains causing two regional outbreaks in Asturias (Spain) were phylogenetically characterized.
STUDY DESIGN
Mumps virus strains, which were detected in samples from patients belonging to two regional outbreaks in Asturias, were characterized by sequencing of the SH gene and further alignment to homologous sequences of representative strains of the different mumps genotypes.
RESULTS
Two different strains (Ast/SP02 and Ast/SP07) were isolated. Sequence analysis revealed that while Ast/SP02 belonged to genotype H, Ast/SP07 was phylogenetically close to UK02-19, a reference strain for a new genotype. Both strains belonged to different genotypes from those used in the vaccination (Jeryl-Lynn strain is genotype A).
CONCLUSION
Mumps virus strains different from those used in vaccination program can cause mumps outbreaks even in vaccinated patients.
背景
尽管进行了普遍接种疫苗，但每年在不同国家仍会检测到几例可能引发疫情的散发性腮腺炎感染病例。
目的
对在西班牙阿斯图里亚斯引发两次地区性疫情的腮腺炎病毒株进行系统发育特征分析。
研究设计
对从阿斯图里亚斯两次地区性疫情患者样本中检测到的腮腺炎病毒株，通过对SH基因进行测序并与不同腮腺炎基因型代表性毒株的同源序列进一步比对来进行特征分析。
结果
分离出两种不同的毒株（Ast/SP02和Ast/SP07）。序列分析显示，Ast/SP02属于H基因型，而Ast/SP07在系统发育上与UK02 - 19接近，UK02 - 19是一种新基因型的参考毒株。这两种毒株与疫苗中使用的毒株（Jeryl - Lynn毒株是A基因型）属于不同的基因型。
结论
即使在接种过疫苗的患者中，与疫苗中使用的毒株不同的腮腺炎病毒株也可能引发腮腺炎疫情。
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