Kasai N, Ogassawara S, Baccaro M R
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo.
Rev Bras Biol. 1991 Nov;51(4):731-45.
The life cycle of a species of murine Eimeria, isolated from a commercial source of mice is described using experimental infections. A pure strain was established starting from a single oocyst administered to Eimeria-free Swiss white mice. The prepatent period was 6 1/2 to 7 days, and the patent period lasted 6 to 10 days. Oocysts were subspherical or ovoid, with a smooth double-layered colourless membrane. Micropila was absent. The sporulated oocysts measured 15.7-25.1 x 14.1-22.0 microns (X780 = 21.3 x 16.9 microns). One to 4 polar granules were present. The oocyst residuum was absent. Sporocysts were ellipsoid, measured 11.5 x 6.3 microns (n = 780), with Stieda body and a compact subspherical sporocyst residuum. The sporozoites were elongated, 17.7 x 3.2 microns (n = 100), partially curled around each other. The endogenous stages were found only in the large intestine, with at least 4 generations of schizonts. Mature 1st generation schizonts, first seen 24 hr post-infection (HPI) measured 10.3 x 10.6 microns and had 8 to 25 merozoites 5.0-10.0 x 1.0-3.0 microns. Mature 2nd generation schizonts were first seen 48 HPI, measured 14.4 x 8.1 microns and had 8 to 20 merozoites 11.0-16.0 x 2.0-3.0 microns. Two forms of presumably 3rd generation schizonts appeared between 72 and 96 HPI: one measuring 13.9 x 8.5 microns, containing large merozoites (17.0-19.0 x 2.0-4.0 microns) and the other measuring 7.3 x 10.4 microns, where 6 to 10 merozoites (8.0-13.0 x 1.0-3.0 microns) were found. Fourth generation schizonts measuring 10.4 x 11.9 microns, with 26 a 48 or more merozoites (8.0-13.0 x 2.0-3.0 microns) were first seen at 132 HPI. Mature macro and microgamonts were first observed at 144 HPI. The sporulation of 72.0% of the oocysts in 2.0% (w/v) potassium dichromate at 23-24 degrees C occurred in 9 days. Usually the infected mice showed depression, anorexia, weight loss, diarrhea or dysentery, and dehydration. The clinical picture w as more pronounced at 6 to 10 days post-infection (DPI). Mortality was highest between the 8th and 13th DPI, with a peak at 9 DPI in groups which received 1.1 x 10(4) to 4.4 x 10(4) oocysts. Groups infected with 5.5 x 10(3), 8.8 x 10(4) or 1.76 x 10(5) oocysts showed different results. Epithelial destruction, edema, inflammation, necrotizing enteritis followed by glandular regeneration and realing were observed restricted to the large intestine. The isolated Eimeria was named E. falciformis var. pragensis based on its morphobiological characteristics.
通过实验性感染，描述了从商业来源小鼠中分离出的一种鼠艾美耳球虫的生命周期。从接种给无艾美耳球虫的瑞士小白鼠的单个卵囊开始建立了一个纯菌株。潜隐期为6.5至7天，寄生期持续6至10天。卵囊呈亚球形或卵形，有光滑的双层无色膜。无微孔。孢子化卵囊大小为15.7 - 25.1×14.1 - 22.0微米（X780 = 21.3×16.9微米）。有1至4个极粒。无卵囊残体。孢子囊呈椭圆形，大小为11.5×6.3微米（n = 780），有斯氏体和紧密的亚球形孢子囊残体。子孢子细长，17.7×3.2微米（n = 100），部分相互卷曲。内源性发育阶段仅在大肠中发现，至少有4代裂殖体。成熟的第一代裂殖体在感染后24小时首次见到，大小为10.3×10.6微米，有8至25个裂殖子，大小为5.0 - 10.0×1.0 - 3.0微米。成熟的第二代裂殖体在感染后48小时首次见到，大小为14.4×8.1微米，有8至20个裂殖子，大小为11.0 - 16.0×2.0 - 3.0微米。在感染后72至96小时出现两种可能的第三代裂殖体：一种大小为13.9×8.5微米，含有大裂殖子（17.0 - 19.0×2.0 - 4.0微米），另一种大小为7.3×10.4微米，有6至10个裂殖子（8.0 - 13.0×1.0 - 3.0微米）。第四代裂殖体大小为10.4×11.9微米，有26至48个或更多裂殖子（8.0 - 13.0×2.0 - 3.0微米），在感染后132小时首次见到。成熟的大配子体和小配子体在感染后144小时首次观察到。72.0%的卵囊在23 - 24℃的2.0%（w/v）重铬酸钾中9天内完成孢子化。通常感染的小鼠表现出抑郁、厌食、体重减轻、腹泻或痢疾以及脱水。临床症状在感染后6至10天更为明显。死亡率在感染后第8至13天最高，在接受1.1×10⁴至4.4×10⁴个卵囊的组中，在感染后第9天达到峰值。感染5.5×10³、8.8×10⁴或1.76×10⁵个卵囊的组显示出不同的结果。观察到上皮破坏、水肿、炎症、坏死性肠炎，随后是腺体再生和修复，这些仅局限于大肠。根据其形态生物学特征，分离出的艾美耳球虫被命名为镰形艾美耳球虫布拉格变种。
