免疫磁珠分离结合实时逆转录聚合酶链反应检测受污染食品中的诺如病毒。
Immunomagnetic separation combined with real-time reverse transcriptase PCR assays for detection of norovirus in contaminated food.
作者信息
Park YoungBin, Cho You-Hee, Jee YoungMee, Ko GwangPyo
机构信息
Department of Environmental Health, School of Public Health, Seoul National University, 28 Yunkeun-Dong, Chongro-Ku, Seoul 110-799, South Korea.
出版信息
Appl Environ Microbiol. 2008 Jul;74(13):4226-30. doi: 10.1128/AEM.00013-08. Epub 2008 Apr 25.
Abstract
We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.
摘要
我们开发了一种免疫磁珠分离（IMS）技术，并结合实时TaqMan逆转录酶聚合酶链反应（RT-PCR），该技术能够从人工污染的草莓中检测到低至3至7个RT-PCR单位水平的诺如病毒。接种物回收率在14%至30%之间。数据表明，IMS与实时RT-PCR相结合将成为检测食源性微生物污染物的一种快速且灵敏的方法。
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