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[哥斯达黎加致病真菌(皮肤真菌科)的形态学和生理学特征]

[Morphologic and physiologic characteristics of Costa Rica pathogenic fungi (Dermatiaceae)].

作者信息

Valiente C, Quesada E

机构信息

Facultad de Microbiología, Universidad de Costa Rica.

出版信息

Rev Biol Trop. 1991 Jun;39(1):103-6.

PMID:1844148
Abstract

Forty one strains of dematiaceous fungi from the Mycology collection of the University of Costa Rica were studied. Thirty three were pathogenic (Fonsecaea pedrosoi, Cladosporium carrionii, Xylohypha bantiana, Exophiala jeanselmei, Rhinocladiella aquaspersa, Phialophora verrucosa) and the other eight were contaminants (Hormodendrum sp.). Morphological studies were done using the slide culture technique. The physiological criteria used were: urease production, gelatin and Loeffler media liquefaction; xanthine, tyrosine, starch and casein hydrolysis; nitrate utilization; carbohydrate uptake; sensitivity to cycloheximide and thermotolerance in glucose-Sabouraud medium. The physiological tests did not provide characteristic patterns for the different genera of pathogenic fungi, even though these differences were detected in non pathogenic fungi; the tests may be useful for the quick separation of both groups. Physiological test may have a limited value in the identification of fungi and the morphological analysis cannot be substituted by physiological studies.

摘要

对来自哥斯达黎加大学真菌学收藏的41株暗色真菌进行了研究。其中33株具有致病性(佩德罗霉、卡氏枝孢霉、班替木丝霉、甄氏外瓶霉、水栖枝顶孢、疣状瓶霉),另外8株为污染物(分支孢霉属)。使用玻片培养技术进行形态学研究。所采用的生理学标准包括:脲酶产生、明胶和吕氏培养基液化;黄嘌呤、酪氨酸、淀粉和酪蛋白水解;硝酸盐利用;碳水化合物摄取;对放线菌酮的敏感性以及在葡萄糖-沙氏培养基中的耐热性。生理学测试并未为致病真菌的不同属提供特征性模式,尽管在非致病真菌中检测到了这些差异;这些测试可能有助于快速区分这两组真菌。生理学测试在真菌鉴定中的价值可能有限,形态学分析不能被生理学研究所替代。

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