Nanne Echandi C I, Aragón Ortiz F
Departamento de Bioquímica, Escuela de Medicina, Universidad de Costa Rica.
Rev Biol Trop. 1991 Jun;39(1):15-21.
A lectin was isolated from the crude extract prepared from the seeds of E. costaricensis. It was purified by gel filtration on Sephadex G-100 followed by DEAE-Sephadex A-50 chromatography. PAGE revealed only one protein band, while analytical isoelectric focusing revealed four bands. The protein is a dimer with M(r) 58kda not united by disulfide bridges. It is a glycoprotein with 6.5% of neutral sugars, stable at 70 degrees C and at a pH range between 2 to 10. The protein exhibited a non specific agglutination of human erythrocytes, nevertheless it differentiated between erythrocytes of animal origin, agglutinating those of rabbit and chicken and not those from horse, goat, sheep or rat. Galactose, N-acetyl-D-galactosamine, lactose and EDTA are inhibitors while Ca++ and Mn++ acted as activators of the agglutination. No change in the blood pressure was observed when the lectin was intravenously injected in rats.
从哥斯达黎加猪屎豆种子制备的粗提物中分离出一种凝集素。通过在Sephadex G - 100上进行凝胶过滤，随后用DEAE - Sephadex A - 50进行色谱法纯化。聚丙烯酰胺凝胶电泳（PAGE）仅显示一条蛋白带，而分析等电聚焦显示四条带。该蛋白是一种分子量为58kDa的二聚体，不由二硫键连接。它是一种糖蛋白，含有6.5%的中性糖，在70℃以及pH值在2至10的范围内稳定。该蛋白对人红细胞表现出非特异性凝集，不过它能区分动物来源的红细胞，凝集兔和鸡的红细胞，而不凝集马、山羊、绵羊或大鼠的红细胞。半乳糖、N - 乙酰 - D - 半乳糖胺、乳糖和乙二胺四乙酸（EDTA）是抑制剂，而钙离子（Ca++）和锰离子（Mn++）作为凝集的激活剂。当将该凝集素静脉注射到大鼠体内时，未观察到血压变化。
