He Yan-Yan, Zhang Shu-Yi, Zhu Min, Qian Min, Tan Hong-Yan
Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2007 Dec;25(6):515-7.
Specific antibodies purified from sera of rabbit infected with Toxoplasma gondii were used to immunoscreen the 12-mer and 7-mer phage random peptide libraries. By 3 rounds of screening, 70 clones were picked out randomly to test the specificity and 43 were found positive by ELISA. 23 of the 43 positive clones showing strong reaction in ELISA were picked out for sequencing. Clone A7 was selected as mimic antigen in ELISA test after analysis of the 23 short peptide sequences. 23 out of 47 sera from rabbits infected with Toxoplasma gondii were ELISA positive by A7 mimic antigen with a positive rate of 68.1%, and 10 of 155 sera from healthy persons showed false positive with a specificity of 93.5%. The primary result suggests that mimic antigen may have a potential use in diagnose of toxoplasmosis.
从感染弓形虫的兔血清中纯化得到的特异性抗体，用于对12肽和7肽噬菌体随机肽库进行免疫筛选。经过三轮筛选，随机挑选出70个克隆进行特异性检测，通过酶联免疫吸附测定（ELISA）发现其中43个呈阳性。在ELISA检测中表现出强烈反应的43个阳性克隆中，挑选出23个进行测序。对这23个短肽序列进行分析后，选择克隆A7作为ELISA检测中的模拟抗原。感染弓形虫的47只兔的血清中，有23只通过A7模拟抗原检测呈ELISA阳性，阳性率为68.1%；155名健康人的血清中有10人呈假阳性，特异性为93.5%。初步结果表明，模拟抗原可能在弓形虫病诊断中具有潜在用途。
