Tuppo Lisa, Giangrieco Ivana, Palazzo Paola, Bernardi Maria Livia, Scala Enrico, Carratore Vito, Tamburrini Maurizio, Mari Adriano, Ciardiello M Antonietta
Institute of Protein Biochemistry, CNR, Via Pietro Castellino 111, I-80131 Napoli, Italy.
J Agric Food Chem. 2008 May 28;56(10):3812-7. doi: 10.1021/jf703620m. Epub 2008 Apr 29.
Kiwellin, an allergenic protein formerly isolated from green kiwi fruit, has been identified as the most abundant component of the gold kiwi species. A protein named KiTH, showing a 20 kDa band on reducing SDS-PAGE and 100% identity with the C-terminal region of kiwellin, has been identified in the extract of the ripe green species. In vitro treatment of purified kiwellin with the protease actinidin from green kiwi fruit originated KiTH and kissper, a recently described pore-forming peptide. Primary structure analysis and experimental evidence suggest that kiwellin is a modular protein with two domains. It may undergo in vivo proteolytic processing by actinidin, thus producing KiTH and kissper. When probed with sera recognizing kiwellin from green kiwi fruit, KiTH showed IgE binding, with reactivity levels sometimes different from those of kiwellin. The IgE-binding capacity of kiwellin from gold kiwi fruit appears to be similar to that of the green species.
奇异果素，一种先前从绿奇异果中分离出的变应原蛋白，已被鉴定为金奇异果品种中含量最丰富的成分。在成熟绿奇异果品种的提取物中，已鉴定出一种名为KiTH的蛋白质，它在还原型SDS-PAGE上显示出一条20 kDa的条带，并且与奇异果素的C端区域具有100%的同一性。用绿奇异果中的蛋白酶肌动蛋白酶对纯化的奇异果素进行体外处理，产生了KiTH和吻刺蛋白（一种最近描述的成孔肽）。一级结构分析和实验证据表明，奇异果素是一种具有两个结构域的模块化蛋白质。它可能在体内被肌动蛋白酶进行蛋白水解加工，从而产生KiTH和吻刺蛋白。当用识别绿奇异果奇异果素的血清进行检测时，KiTH显示出IgE结合，其反应水平有时与奇异果素不同。金奇异果奇异果素的IgE结合能力似乎与绿奇异果品种相似。
