结肠癌干细胞的分离与培养。

Isolation and culture of colon cancer stem cells.

作者信息

Cammareri Patrizia, Lombardo Ylenia, Francipane Maria Giovanna, Bonventre Sebastino, Todaro Matilde, Stassi Giorgio

机构信息

Department of GENURTO, University of Palermo, 129-90127 Palermo, Italy.

出版信息

Methods Cell Biol. 2008;86:311-24. doi: 10.1016/S0091-679X(08)00014-9.

DOI:10.1016/S0091-679X(08)00014-9

PMID:18442654

Abstract

Cancer stem cells (CSCs) resemble normal stem cells in several ways. Both cell types are self-renewing and when they divide, one of the daughter cells differentiates while the other retains stem cell properties, including the ability to divide in the same way again. CSCs have been demonstrated to exist in several solid tumors, including colon carcinoma; these cells are able to initiate and sustain tumor growth. There are essentially three different methods to isolate CSCs: establishment culture, the MACS (magnetic cell sorting) technology, and the FACS (fluorescence-activated cell sorting) technology.

摘要

癌症干细胞（CSCs）在几个方面类似于正常干细胞。这两种细胞类型都具有自我更新能力，并且当它们分裂时，其中一个子细胞会分化，而另一个则保留干细胞特性，包括再次以相同方式分裂的能力。已经证明CSCs存在于包括结肠癌在内的几种实体瘤中；这些细胞能够启动并维持肿瘤生长。基本上有三种不同的方法来分离CSCs：建立培养、磁珠细胞分选（MACS）技术和荧光激活细胞分选（FACS）技术。