Li Xia, Jiao Li-Li, Zhang Xu, Tian Wen-Min, Chen Shan, Zhang Li-Ping
Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Northeast Normal University, Changchun 130024, China.
Int Immunopharmacol. 2008 Jun;8(6):909-15. doi: 10.1016/j.intimp.2008.02.008. Epub 2008 Mar 17.
Two proteoglycans, PNW1 and PNM1, were isolated from the mycelium of Phellinus nigricans through submerged fermentation and culture medium, respectively. PNW1 and PNM1 with similar average molecular weight (33 kDa and 29 kDa) were composed of glucose, galactose, mannose, arabinose and fucose in the molar ratios of 3.26:8.77:6.44:1:1.35 and 20.06:8.72:6.94:1:0.76. At the dose of 100, 200, and 400 mg/kg, PNW1 and PNM1 exhibited anti-tumor activity against mice-transplanted Sarcoma 180 in vivo. However, no direct cytotoxic activity against Sarcoma 180 could be determined. Significant increase in the relative spleen and thymus weight and expression of tumor necrosis factor-alpha (TNF-alpha) in serum was observed, decreasing the tumor weight significantly. PNW1 and PNM1 could stimulate lymphocytes proliferation and increase production of nitric oxide (NO) and TNF-alpha in macrophages. The results indicate that both lymphocyte and macrophages were activated by preparations of proteoglycans from mycelium and culture medium of P. nigricans. The anti-tumor effect of the proteoglycans is not directly tumoricidal but rather immunostimulating.
分别通过深层发酵和培养基从黑柄炭角菌的菌丝体中分离出两种蛋白聚糖，即PNW1和PNM1。平均分子量相似（分别为33 kDa和29 kDa）的PNW1和PNM1由葡萄糖、半乳糖、甘露糖、阿拉伯糖和岩藻糖组成，其摩尔比分别为3.26:8.77:6.44:1:1.35和20.06:8.72:6.94:1:0.76。在剂量为100、200和400 mg/kg时，PNW1和PNM1在体内对小鼠移植的肉瘤180表现出抗肿瘤活性。然而，未确定对肉瘤180有直接的细胞毒性活性。观察到脾脏和胸腺相对重量显著增加以及血清中肿瘤坏死因子-α（TNF-α）的表达增加，肿瘤重量显著降低。PNW1和PNM1可刺激淋巴细胞增殖，并增加巨噬细胞中一氧化氮（NO）和TNF-α的产生。结果表明，黑柄炭角菌菌丝体和培养基中的蛋白聚糖制剂激活了淋巴细胞和巨噬细胞。蛋白聚糖的抗肿瘤作用并非直接杀肿瘤，而是具有免疫刺激作用。
