Li Xia, Jiao LiLi, Zhang Xu, Tian WenMin, Chen Shan, Zhang LiPing
School of Life Sciences, Northeast Normal University, Changchun 130024, China.
Sci China C Life Sci. 2008 Jun;51(6):513-9. doi: 10.1007/s11427-008-0065-1. Epub 2008 May 17.
Two water-soluble polysaccharides, PNW1 and PNM1, were respectively isolated from the mycelium and its culture medium of Phellinus nigricans using submerged fermentation before determining their effects on inhibiting the growth of transplantable tumors in mice. The results of the pharmaceutical experiments showed that oral administration of PNW1 and PNM1 (at a dose of 400 mg/kg) inhibited the growth of tumor of mice-transplanted Sarcoma 180 in vivo. Moreover, a higher inhibition ratio of PNW1 (74.70%) was obtained as compared with PNM1 (55.84%). The averaged molecular weight of PNW1 and PNM1 was determined to be 33 and 29 kD, respectively. Both PNW1 and PNM1 were consisted of glucose, galactose, mannose, arabinose and fucose. The major structural features of PNW1 and PNM1 were elucidated using partial acid hydrolysis, periodate oxidation, Smith degradation, 13C-NMR, methylation and GC-MS. On the basis of these results, the repeating units of PNW1 and PNM1 were established.
采用深层发酵法，分别从黑柄炭角菌的菌丝体及其培养基中分离得到两种水溶性多糖PNW1和PNM1，然后测定它们对小鼠移植性肿瘤生长的抑制作用。药理实验结果表明，口服PNW1和PNM1（剂量为400mg/kg）可在体内抑制小鼠移植肉瘤180的肿瘤生长。此外，与PNM1（55.84%）相比，PNW1的抑制率更高（74.70%）。测定PNW1和PNM1的平均分子量分别为33kD和29kD。PNW1和PNM1均由葡萄糖、半乳糖、甘露糖、阿拉伯糖和岩藻糖组成。采用部分酸水解、高碘酸盐氧化、Smith降解、13C-NMR、甲基化和GC-MS等方法阐明了PNW1和PNM1的主要结构特征。基于这些结果，确定了PNW1和PNM1的重复单元。
