[用于诊断伤寒热的免疫酶技术（酶联免疫吸附测定法）的评估]

[Evaluation of an immunoenzyme technique (ELISA) to diagnose typhoid fever].

作者信息

Figueroa G, Jashés M, Fáundez G, Toledo M S, Troncoso M, Aguad L

机构信息

Unidad de Microbiología, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago de Chile.

出版信息

Rev Med Chil. 1991 May;119(5):506-11.

PMID:1844287

Abstract

The efficiency of an ELISA method, designed to detect polyvalent IgG and IgM antibodies to Salmonella typhi polysaccharide was evaluated in patients admitted or convalescing from typhoid fever and in control subjects. Polyvalent antibodies to S typhi were demonstrated in 28/30 (93%) typhoid patients, 0/11 bacteremic patients (E coli or S paratyphi A) and 0/15 asymptomatic individuals. Widal test showed significant anti-0 agglutinin values (> = 1: 160) in only 12/30 (40%), 1/11 (9%) and 0/15 subjects from each group respectively. Typhoid patients tested on admission or at discharge showed similar high reactivity rates to ELISA. On the contrary, the Widal test detected only 2/15 (13%) patients on admission (p < 0.02) and 10/15 (67%) at discharge. These results and additional immunoblotting tests suggest that ELISA developed to detect polyvalent anti-LPS antibodies could represent a highly specific diagnostic tool to confirm typhoid fever in endemic areas.

摘要

在伤寒热患者（包括正在住院或处于恢复期的患者）及对照受试者中，评估了一种用于检测抗伤寒沙门氏菌多糖多价IgG和IgM抗体的ELISA方法的效率。在30例伤寒患者中有28例（93%）检测到抗伤寒沙门氏菌多价抗体，11例菌血症患者（大肠杆菌或甲型副伤寒沙门氏菌）中无1例检测到，15例无症状个体中也无1例检测到。肥达试验分别在每组的12/30（40%）、1/11（9%）和0/15受试者中显示出显著的抗O凝集素值（≥1:160）。入院时或出院时检测的伤寒患者对ELISA显示出相似的高反应率。相反，肥达试验在入院时仅检测到2/15（13%）的患者（p<0.02），在出院时检测到10/15（67%）的患者。这些结果以及其他免疫印迹试验表明，开发用于检测多价抗LPS抗体的ELISA可能是一种在流行地区确诊伤寒热的高度特异性诊断工具。