Nardiello S, Digilio L, Leva E, Pizzella T, Galanti B
Laboratorio di Immunologia Clinica, I Facoltà di Medicina, Università di Napoli.
Boll Ist Sieroter Milan. 1989;68(3):234-40.
We have evaluated several commercial monoclonal antibodies, specific for human IgG subclasses, for their reactivity in an ELISA test for the characterization of subclasses of IgG anti- Salmonella typhi lipopolysaccharide (LPS) antibodies. Four monoclonals, each specific for a single IgG subclass, were chosen for their good reactivity. In 19 typhoid and non-typhoid serum samples, the sum of the ELISA values obtained with the four subclass-specific monoclonals was highly correlated with the ELISA values obtained with a monoclonal anti-total IgG. Moreover, there was no competition among the various IgG subclasses of anti-LPS antibodies. These data indicate that the subclass distribution of IgG anti-LPS antibodies, calculated on the basis of ELISA values in the subclass-specific assays, is likely to reflect the actual distribution of the different subclasses in whole serum. Different subclass patterns of IgG anti-LPS antibodies were observed in serum samples from 11 patients with typhoid fever, with IgG1 and IgG2 being the most represented subclasses. The ELISA method described here will be useful to elucidate the factors that influence the anti-LPS subclass profile during the humoral immune response to Salmonella typhi.
我们评估了几种针对人IgG亚类的商用单克隆抗体，以检测它们在酶联免疫吸附测定（ELISA）中对IgG抗伤寒杆菌脂多糖（LPS）抗体亚类进行鉴定时的反应性。选择了四种分别针对单一IgG亚类的单克隆抗体，因其反应性良好。在19份伤寒和非伤寒血清样本中，用这四种亚类特异性单克隆抗体获得的ELISA值总和与用抗总IgG单克隆抗体获得的ELISA值高度相关。此外，抗LPS抗体的各种IgG亚类之间不存在竞争。这些数据表明，基于亚类特异性检测中的ELISA值计算得出的IgG抗LPS抗体的亚类分布，可能反映了全血清中不同亚类的实际分布。在11例伤寒热患者的血清样本中观察到了IgG抗LPS抗体的不同亚类模式，其中IgG1和IgG2是最主要的亚类。本文所述的ELISA方法将有助于阐明在对伤寒杆菌的体液免疫反应中影响抗LPS亚类谱的因素。
