Réz G, Pálfia Z, Fellinger E
Department of General Zoology, Eötvös Loránd University, Budapest, Hungary.
Acta Biol Hung. 1991;42(1-3):133-40.
While investigating the time course of the influence of autophagy-inducer vinblastine in pancreatic acinar cells, we discovered that about 24 h after the single injection of the drug (10 mg/kg b.wt.) when most of the cells were already recovering from the autophagic wave, dying cells with apoptotic nuclei and peculiar morphology of their cytoplasm appeared in the exocrine pancreas. Apoptotic blebs (ABs) separating from apoptotic cells or already phagocytized by neighbouring cells were also also observed. A large number of autophagic vacuoles (AVs) were seen in these cells termed apoptotic cell (AC). They are most probably derived from cells with morphologically normal nuclei but with unusually high number of AVs in their cytoplasm. We termed these cells highly autophagic cells (HAC). Our morphometric measurements show that the partial volume of both HAC and AC increased to 7.5 and 9.5%, respectively, of total cellular volume in the 25th h after vinblastine treatment. This increase could be inhibited by a treatment at the 24th h with cycloheximide (0.2 mg/g b.wt.) an inhibitor of both translation and autophagic segregation. Thus, synthesis of proteins or an enhanced autophagy may be indispensable step(s) in the apoptotic process in this system.
在研究自噬诱导剂长春碱对胰腺腺泡细胞影响的时间进程时,我们发现单次注射该药物(10 mg/kg体重)约24小时后,当大多数细胞已从自噬波中恢复时,外分泌胰腺中出现了具有凋亡细胞核和特殊细胞质形态的死亡细胞。还观察到从凋亡细胞分离或已被相邻细胞吞噬的凋亡小泡(ABs)。在这些被称为凋亡细胞(AC)的细胞中可见大量自噬泡(AVs)。它们很可能源自细胞核形态正常但细胞质中自噬泡数量异常高的细胞。我们将这些细胞称为高度自噬细胞(HAC)。我们的形态计量学测量表明,长春碱处理后第25小时,HAC和AC的部分体积分别增加到细胞总体积的7.5%和9.5%。在第24小时用环己酰亚胺(0.2 mg/g体重)处理可抑制这种增加,环己酰亚胺是翻译和自噬分离的抑制剂。因此,蛋白质合成或增强的自噬可能是该系统凋亡过程中不可或缺的步骤。