Tímár J, Paterson H
1st Institute of Pathology and Experimental Cancer Research, Semmelweis Medical University, Budapest, Hungary.
Acta Microbiol Hung. 1991;39(4):259-65.
The cellular localization of the p21 protein was studied by immunoelectron microscopy in human fibrosarcoma cells with different N-ras gene expression. The tumorigenic HT1080 cells--expressing equally the normal and mutant N-ras genes--contained the p21 in the plasma membrane, in pinocytotic areas as well as in the intracellular vesicles. The non-tumorigenic revertants--expressing more normal N-ras gene than the mutant one--contained p21 in the plasma membrane, especially at the cell junctions. These results suggest, that the mutant p21 may locate to the membrane areas connected with pino- or endocytotic functions.
利用免疫电子显微镜技术，在具有不同N-ras基因表达的人纤维肉瘤细胞中研究了p21蛋白的细胞定位。致瘤性HT1080细胞（同时表达正常和突变型N-ras基因）的p21存在于质膜、胞饮区域以及细胞内小泡中。非致瘤性回复突变体（表达的正常N-ras基因多于突变型N-ras基因）的p21存在于质膜中，尤其是在细胞连接处。这些结果表明，突变型p21可能定位于与胞饮或内吞功能相关的膜区域。
