Fan Jiang Ping, Kim Han Seop, Han Gi Dong
Department of Food Science and Technology, College of Natural Resources, Yeungnam University, Gyeongsan, Republic of Korea.
Amino Acids. 2009 Feb;36(2):365-72. doi: 10.1007/s00726-008-0093-y. Epub 2008 Apr 29.
This study shows the effects of L-carnitine treatment on cell proliferation with hepa1c1c7 mouse cancer cells and NCTC 1469 normal cells. In an MTT assay, L-carnitine increased the number of dead hepa1c1c7 cells, while there was no difference in the number of NCTC 1469 cells. mRNA and protein levels of TNF-alpha, Fas, and caspase-8, which are closely related to cell apoptosis by a death ligand/receptor-dependent apoptosis pathway, were increased by L-carnitine treatment. In addition, L-carnitine treatment regulated mitochondria-dependent apoptosis pathways by inducing the up-regulation of caspase-9 and caspase-3 and the down-regulation of Bcl-2 in hepa1c1c 7 cells. Taken together, the findings of this study have demonstrated that L-carnitine could induce apoptosis in hepa1c1c7 cells by regulating Fas ligands and inhibiting the expression of Bcl-2. These results suggest that L: -carnitine treatment could be related to both a mitochondrion-dependent and a death ligand/receptor-dependent apoptosis pathway in hepa1c1c7 cells. Our results could give information for understanding the L-carnitine-induced apoptosis mechanism in some cancer cells.
本研究显示了L-肉碱处理对hepa1c1c7小鼠癌细胞和NCTC 1469正常细胞增殖的影响。在MTT试验中，L-肉碱增加了hepa1c1c7死亡细胞的数量，而NCTC 1469细胞数量没有差异。L-肉碱处理增加了与死亡配体/受体依赖性凋亡途径密切相关的TNF-α、Fas和caspase-8的mRNA和蛋白质水平。此外，L-肉碱处理通过诱导hepa1c1c7细胞中caspase-9和caspase-3的上调以及Bcl-2的下调来调节线粒体依赖性凋亡途径。综上所述，本研究结果表明，L-肉碱可通过调节Fas配体和抑制Bcl-2的表达诱导hepa1c1c7细胞凋亡。这些结果表明，L-肉碱处理可能与hepa1c1c7细胞中线粒体依赖性和死亡配体/受体依赖性凋亡途径有关。我们的结果可为理解L-肉碱诱导某些癌细胞凋亡的机制提供信息。
