Lazar E E, Wills R B H, Ho B T, Harris A M, Spohr L J
Gosford Horticultural Institute, NSW Department of Primary Industries, Gosford, NSW, Australia.
Lett Appl Microbiol. 2008 Jun;46(6):688-92. doi: 10.1111/j.1472-765X.2008.02373.x. Epub 2008 Apr 28.
To evaluate the antifungal activity of nitric oxide (NO) against the growth of the postharvest horticulture pathogens Aspergillus niger, Monilinia fructicola and Penicillium italicum under in vitro conditions.
Different volumes of NO gas were injected into the Petri dish headspace to obtain the desired concentrations of 50-500 microl l(-1). The growth of the fungi was measured for 8 days of incubation in air at 25 degrees C. All concentrations of NO were found to produce an antifungal effect on spore germination, sporulation and mycelial growth of the three fungi, with the most effective concentration for A. niger and P. italicum being 100 and 500 microl l(-1) for M. fructicola.
Short-term exposure to a low concentration of NO gas was able to inhibit the subsequent growth of A. niger, M. fructicola and P. italicum.
NO gas has potential use as a natural fungicide to inhibit microbial growth on postharvest fruit and vegetables.
评估一氧化氮（NO）在体外条件下对采后园艺病原菌黑曲霉、褐腐病菌和意大利青霉生长的抗真菌活性。
向培养皿顶空注入不同体积的NO气体，以获得50 - 500微升/升的所需浓度。在25℃空气中培养8天，测定真菌的生长情况。发现所有浓度的NO对三种真菌的孢子萌发、产孢和菌丝生长均产生抗真菌作用，对黑曲霉和意大利青霉最有效的浓度为100微升/升，对褐腐病菌为500微升/升。
短期暴露于低浓度NO气体能够抑制黑曲霉、褐腐病菌和意大利青霉的后续生长。
NO气体有作为天然杀菌剂抑制采后果蔬上微生物生长的潜在用途。
