Labuda R, Piovarciová Z, Tancinová D
Biopure Referenzsubstanzen GmbH, Technopark 1, 3430 Tulln, Austria.
Lett Appl Microbiol. 2008 Jun;46(6):673-5. doi: 10.1111/j.1472-765X.2008.02370.x. Epub 2008 Apr 25.
A possibility of using cycloheximide tolerance and/or sensitivity as an additional diagnostic tool for distinguishing morphologically related species within common small-spored Alternaria has been tested during this study.
A total of 33 strains from four Alternaria species-groups, namely Alternaria alternata, Alternaria arborescens, Alternaria infectoria and Alternaria tenuissima were tested for their growth response to 100 microg m(-1) cycloheximide in potato carrot agar. All A. infectoria strains were completely inhibited, showing no growth at all even after prolonged incubation. In contrast, all other strains representing the remnant three species exhibited a high resistance to this antibiotic.
Cycloheximide sensitivity represents a further important physiological character for distinguishing A. infectoria from the three similar species.
The relevance of these findings corresponds with the potential ability of the Alternaria species produce mycotoxins. Cycloheximide may be in future used in the design of selective media for the isolation of some potentially toxigenic food-borne Alternaria species such as A. alternata, A. tenuissima and/or A. arborescens, for example in screening cereals for toxigenic Alternaria spp. and for their direct separation from nontoxigenic representatives of A. infectoria species-group.
在本研究中测试了使用环己酰亚胺耐受性和/或敏感性作为一种额外的诊断工具，以区分常见小孢子链格孢属内形态相关物种的可能性。
对来自四个链格孢属物种组的33个菌株进行了测试，这四个物种组分别是链格孢、树状链格孢、侵染链格孢和细极链格孢，检测它们在马铃薯胡萝卜琼脂培养基中对100微克/毫升环己酰亚胺的生长反应。所有侵染链格孢菌株均被完全抑制，即使长时间培养也完全不生长。相比之下，代表其余三个物种的所有其他菌株对这种抗生素表现出高度抗性。
环己酰亚胺敏感性是区分侵染链格孢与其他三个相似物种的另一个重要生理特征。
这些发现的相关性与链格孢属物种产生霉菌毒素的潜在能力相对应。环己酰亚胺未来可能用于设计选择性培养基，以分离一些潜在产毒的食源性链格孢属物种，如链格孢、细极链格孢和/或树状链格孢，例如用于筛选谷物中的产毒链格孢属物种，并将它们与侵染链格孢物种组的非产毒代表直接分离。
