Han H-M, Zuber B, Dubochet J
Laboratoire d'Analyse Ultrastructurale, Bâtiment de Biologie, Université de Lausanne, CH-1015 Lausanne, Switzerland.
J Microsc. 2008 May;230(Pt 2):167-71. doi: 10.1111/j.1365-2818.2008.01972.x.
Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.
压缩和裂隙是玻璃体切片冷冻超薄切片术中常见的切割伪像。在最佳切割条件下，它们可以减少或抑制。在本研究中，测量了不同切割速度和刀角下的压缩和厚度。发现压缩随进给量减小，并且裂隙仅在一定厚度以上出现。玻璃体切片的最佳进给量在50至80纳米之间。通过两种独立方法计算的厚度与进给量和压缩定量相关。
