Grubbs R D, Beltz P A, Koss K L
Department of Pharmacology and Toxicology, Wright State University, Dayton, Ohio.
Magnes Trace Elem. 1991;10(2-4):142-50.
The development of fluorescent ion-selective indicators for magnesium has provided valuable tools for measuring second-by-second changes in cytosolic magnesium activity. In the course of establishing appropriate protocols for using one of these indicators, mag-fura-2, to measure magnesium activity in BC3H-1 cells and chick ventricular myocytes, many potential pitfalls and limitations of this technique have been encountered and addressed. These observations are presented for the purpose of providing guidelines regarding the appropriate use of this indicator and on factors influencing the interpretation of resulting data.
用于镁离子的荧光离子选择性指示剂的开发为测量细胞溶质镁活性的逐秒变化提供了有价值的工具。在建立使用其中一种指示剂镁荧光素-2来测量BC3H-1细胞和鸡心室肌细胞中镁活性的合适方案的过程中，已经遇到并解决了该技术的许多潜在陷阱和局限性。呈现这些观察结果的目的是提供关于该指示剂正确使用以及影响所得数据解释的因素的指导方针。
