Sung Woo Sang, Lee Juneyoung, Lee Dong Gun
School of Life Sciences & Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Republic of Korea.
Biochem Biophys Res Commun. 2008 Jul 4;371(3):551-5. doi: 10.1016/j.bbrc.2008.04.107. Epub 2008 Apr 28.
Piscidin 2 (P2), a 22-residue cationic peptide isolated from the mast cells of hybrid striped bass, has potent antibacterial activities. However, its antifungal properties are not completely understood. In the current study, we investigated the antifungal effects and mode of action of P2. P2 exhibited potent antifungal activity against human pathogenic fungi. To understand the fungicidal properties of P2, we focused on a membrane-active mechanism of the peptide by in vivo and in vitro testing. Flow cytometric analysis using bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC(4)(3)] and protoplast regeneration experiments showed that P2 caused fungal membrane damage. Furthermore, fluorescence analysis using 1,6-diphenyl-1,3,5-hexatriene (DPH) revealed that P2 created pores in fungal membranes. These results were confirmed with dye leakage tests by using liposomes composed of phosphatidylcholine/phosphatidylserine (3:1, w/w), which mimicked fungal membranes. The present study indicated that P2 exerts its fungicidal effects by perturbing membrane activities.
杀鱼菌素2（P2）是一种从杂交条纹鲈肥大细胞中分离出的由22个氨基酸残基组成的阳离子肽，具有强大的抗菌活性。然而，其抗真菌特性尚未完全明确。在本研究中，我们探究了P2的抗真菌作用及其作用方式。P2对人类致病真菌表现出强大的抗真菌活性。为了解P2的杀菌特性，我们通过体内和体外试验着重研究了该肽的膜活性机制。使用双（1,3 - 二丁基巴比妥酸）三甲川氧杂羰花青[DiBAC(4)(3)]进行的流式细胞术分析和原生质体再生实验表明，P2会导致真菌细胞膜损伤。此外，使用1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）进行的荧光分析显示，P2会在真菌细胞膜上形成孔洞。通过使用由磷脂酰胆碱/磷脂酰丝氨酸（3:1，w/w）组成的模拟真菌细胞膜的脂质体进行的染料泄漏试验证实了这些结果。本研究表明，P2通过干扰膜活性发挥其杀菌作用。
