Influence of 3-indoleacetic acid on cytochemical changes in nuclei and cytoplasm of human fibroblasts in the cell culture.

The performed studies covered the action of 3-indoleacetic acid (3IAA) on human fibroblasts cultured in vitro. The 3IAA implemented in the studies was provided by Chemapol Firm (Czechoslovakia). All the investigations were carried out on I passage fibroblasts, taken from the human skin in form of monolayer culture. Feulgen's method and autographic one were resorted to in the studies. The optimal time of acid hydrolysis was 10 min. Quantitative measurements of DNA in nuclei of fibroblasts were done on integrating cytophotometer. Incubation with labelled 3H uridine as RNA precursor was employed for demonstrating the activity of cell nucleus transcription. As concerns the quantitative changes in nucleic acids, it was disclosed that DNA and RNA synthesis was intensified. Nuclei were seen to appear with polydiploidal DNA amount in cultures being influenced by 3IAA action. The smallest number of mitoses was revealed in the cultures exposed to the action of the highest dose of the said compound. Stronger 3H uridine incorporation was recorded in cultures 24 and 72 hours after the administration of 10 mg/1000 ml of 3IAA. The results were presented in the form of diagrams, nucleinogram and tables.