Baby Padmini S, Appala Raju B, Mani K R
School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore-632 014, Tamil Nadu, India.
Indian J Med Microbiol. 2008 Apr-Jun;26(2):163-6. doi: 10.4103/0255-0857.40534.
A total of 23 clinical isolates (15 Escherichia coli and 8 Klebsiella pneumoniae), resistant to cefotaxime and ceftazidime recovered during 2002 and 2003, were investigated for production of CTX-M extended spectrum beta-lactamase (ESBL) by phenotypic and molecular methods. The presence of ESBL was tested by NCCLS phenotypic confirmatory test using cephalosporin/clavulanate combination discs and E-test ESBL strips. Determination of MIC of cefotaxime and ceftazidime was done with and without the presence of clavulanic acid by agar dilution technique. Polymerase chain reaction revealed the presence of CTX-M type ESBLs in 19 isolates. Further sequencing resulted in identification of CTX-M-15 ESBLs. This is the first report identifying CTX-M type ESBL from clinical isolates of E. coli and K. pneumoniae from a tertiary care hospital in south India.
2002年至2003年期间，共回收了23株对头孢噻肟和头孢他啶耐药的临床分离株（15株大肠杆菌和8株肺炎克雷伯菌），通过表型和分子方法对其CTX-M超广谱β-内酰胺酶（ESBL）的产生情况进行了研究。使用头孢菌素/克拉维酸组合药敏纸片和E-test ESBL试纸条，通过NCCLS表型确证试验检测ESBL的存在。采用琼脂稀释法，在有和没有克拉维酸存在的情况下测定头孢噻肟和头孢他啶的最低抑菌浓度（MIC）。聚合酶链反应显示19株分离株中存在CTX-M型ESBL。进一步测序鉴定出CTX-M-15型ESBL。这是首次从印度南部一家三级护理医院的大肠杆菌和肺炎克雷伯菌临床分离株中鉴定出CTX-M型ESBL的报告。
