Kleine Lauren G, Solano Mauricio, Rusckowski Mary, Hunt Kathleen E, Johnson Karen L, Kirker-Head Carl A
Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA.
Am J Vet Res. 2008 May;69(5):639-46. doi: 10.2460/ajvr.69.5.639.
To evaluate the use of technetium Tc 99m-labeled EDTA-biotin monomer ((99m)Tc-EB1) as a scintigraphic imaging agent for soft tissue inflammatory lesions in horses.
6 healthy adult horses.
First (phase 1), the agent's safety and blood-tissue clearance and an appropriate imaging protocol were determined in 6 horses. Each horse was injected with (99m)Tc-EB1 (1.1 GBq, IV, once); images were acquired at intervals during the following 24-hour period. Subsequently (phase 2), inflammation was induced via injection of 200 mg (10 mL) of mepivacaine (0.4 mg/kg) into the right neck musculature and perineurally in the proximal palmar metacarpal region of the right forelimb of 2 horses. Six hours after mepivacaine injection, (99m)Tc-EB1 (2.2 GBq, IV, once) was administered; 8 hours after injection, comparative soft tissue images were acquired after administration of technetium (99m)Tc-hydroxymethylene diphosphonate ((99m)Tc-HDP; 7.4 GBq, IV, once).
After injections of (99m)Tc-EB1, physical examinations, CBCs, and serum biochemical analyses revealed no abnormalities in any horse. Blood clearance of (99m)Tc-EB1 was rapid (A phase, 2.2 minutes; beta phase, 58 minutes). Soft tissue uptake of (99m)Tc-EB1 was immediate and persisted for as long as 4 hours after injection. At 6 hours after IM and perineural mepivacaine injections, mepivacaine-induced inflammation was detectable by use of (99m)Tc-EB1.
Results indicated that (99m)Tc-EB1 is safe for use in horses and can identify soft tissue inflammation without concurrent uptake in bone. Compared with (99m)Tc-HDP administration, use of (99m)Tc-EB1 extended the duration of soft tissue scintigraphic image acquisition.
评估锝 Tc 99m 标记的乙二胺四乙酸 - 生物素单体（(99m)Tc - EB1）作为马匹软组织炎性病变闪烁成像剂的应用。
6 匹健康成年马。
首先（第 1 阶段），在 6 匹马中确定该药剂的安全性、血液 - 组织清除率及合适的成像方案。每匹马静脉注射(99m)Tc - EB1（1.1 GBq，一次）；在随后的 24 小时内间隔采集图像。随后（第 2 阶段），通过向 2 匹马右颈部肌肉组织及右前肢近端掌侧掌骨区域神经周围注射 200 mg（10 mL）甲哌卡因（0.4 mg/kg）诱导炎症。甲哌卡因注射 6 小时后，静脉注射(99m)Tc - EB1（2.2 GBq，一次）；注射 8 小时后，在静脉注射锝(99m)Tc - 羟亚甲基二膦酸盐（(99m)Tc - HDP；7.4 GBq，一次）后采集对比软组织图像。
注射(99m)Tc - EB1 后，体格检查、全血细胞计数及血清生化分析显示所有马匹均无异常。(99m)Tc - EB1 的血液清除迅速（A 期，2.2 分钟；β期，58 分钟）。(99m)Tc - EB1 在软组织中的摄取即刻发生，且注射后可持续长达 4 小时。在肌肉注射和神经周围注射甲哌卡因 6 小时后，使用(99m)Tc - EB1 可检测到甲哌卡因诱导的炎症。
结果表明(99m)Tc - EB1 用于马匹是安全的，并且能够识别软组织炎症而不会同时摄取到骨骼中。与注射(99m)Tc - HDP 相比，使用(99m)Tc - EB1 延长了软组织闪烁成像图像采集的持续时间。
