Department of Pathology, University of Maryland, Baltimore, MD, USA.
Diagn Pathol. 2008 Apr 30;3:21. doi: 10.1186/1746-1596-3-21.
Histologically documented cases of parvoviral myocarditis are exceedingly rare.
Here, we report a 41-year old African American immunocompetent patient who died of parvoviral myocarditis after a 10 day illness characterized by fever, headaches, generalized arthralgias, and a maculopapular rash.Autopsy revealed an infiltrate myocarditis composed primarily of T-lymphocytes and macrophages associated with extensive myocardial fibrosis. The diagnosis of parvovirus was determined by polymerase chain reaction (PCR) on both pre-mortem serum and post-mortem myocardial tissue
DNA was extracted from tissue and serum and primers were used to amplify DNA sequences of parvovirus B19 using nested polymerase chain reaction (PCR).
The diagnosis of parvovirus should be considered in cases of fatal myocarditis, and diagnosis can be confirmed at autopsy by molecular techniques.
组织学证实的细小病毒心肌炎极为罕见。
这里，我们报告了一例 41 岁非裔美国免疫功能正常患者，在发热、头痛、全身关节痛和斑丘疹 10 天后死于细小病毒性心肌炎。尸检显示心肌炎浸润主要由 T 淋巴细胞和巨噬细胞组成，伴有广泛的心肌纤维化。聚合酶链反应 (PCR) 同时检测生前血清和死后心肌组织确定了细小病毒 B19 的诊断。
从组织和血清中提取 DNA，使用巢式聚合酶链反应 (PCR) 扩增细小病毒 B19 的 DNA 序列。
在致命性心肌炎的病例中应考虑细小病毒的诊断，并且可以通过分子技术在尸检时确诊。
