Chan Barden, Yuan Hai-Tao, Ananth Karumanchi S, Sukhatme Vikas P
Division of Interdisciplinary Medicine and Biotechnology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, RW 563, Boston, MA 02215, USA.
Biochem Biophys Res Commun. 2008 Jul 4;371(3):475-9. doi: 10.1016/j.bbrc.2008.04.091. Epub 2008 Apr 28.
Tie-1 is an endothelial specific cell surface protein whose biology remains poorly understood. Using an overexpression system in vitro, we examined whether Tie-1 activity in endothelial cells in vitro would elicit a proinflammatory response. We found that when overexpressed in endothelial cells in vitro, Tie-1 is tyrosine-phosphorylated. We also showed that Tie-1 upregulates VCAM-1, E-selectin, and ICAM-1, partly through a p38-dependent mechanism. Interestingly, upregulation of VCAM-1 and E-selectin by Tie-1 is significantly higher in human aortic endothelial cells than in human umbilical vein endothelial cells. Additionally, attachment of cells of monocytic lineage to endothelial cells is also enhanced by Tie-1 expression. Collectively, our data show that Tie-1 has a proinflammatory property and may play a role in the endothelial inflammatory diseases such as atherosclerosis.
Tie-1是一种内皮细胞特异性细胞表面蛋白，其生物学特性仍知之甚少。我们利用体外过表达系统，研究了体外内皮细胞中的Tie-1活性是否会引发促炎反应。我们发现，当在体外内皮细胞中过表达时，Tie-1会发生酪氨酸磷酸化。我们还表明，Tie-1部分通过p38依赖机制上调血管细胞黏附分子-1（VCAM-1）、E-选择素和细胞间黏附分子-1（ICAM-1）。有趣的是，Tie-1对VCAM-1和E-选择素的上调在人主动脉内皮细胞中比在人脐静脉内皮细胞中显著更高。此外，Tie-1表达也增强了单核细胞系细胞与内皮细胞的黏附。总体而言，我们的数据表明Tie-1具有促炎特性，可能在动脉粥样硬化等内皮炎症性疾病中起作用。
