Reissig Dieter, Rassoul Fausi, Salvetter Jürgen, Wagner Oswald, Richter Volker
Institute of Anatomy, University Leipzig, Liebigstr. 13, 04103, Leipzig, Germany.
Eur J Nutr. 2003 Aug;42(4):224-7. doi: 10.1007/s00394-003-0408-4.
With respect to linoleic acid both beneficial and proatherogenic effects have been described. However, the effect on expression of cell adhesion molecules on human coronary artery endothelial cells (HCAEC) is not yet established. The aim of the experiments was to evaluate the influence of linoleic acid in comparison with palmitic acid regarding the cytokine-induced expression of endothelial leukocyte adhesion molecules (intercellular cell adhesion molecule-1 ICAM-1, vascular cell adhesion molecule-1 VCAM-1, E-selectin).
HCAEC were cultured in microvascular endothelial cell growth medium. In the experiments, the cells were preincubated with linoleic acid and palmitic acid, respectively (10 micro mol/l, 2 days) or under control conditions, after which interleukin- 1alpha (IL-1alpha, 10 ng/ml in the test medium) was added for 1 day. The monoclonal antibodies used were fluorescein isothiocyanate (FITC)- labeled anti-ICAM-1, FITC-labeled anti-VCAM-1, and FITC-labeled anti-E-selectin. Expression was analyzed by flow cytofluorimetry. Next, to examine the effects of fatty acids on adhesion of monocytes to endothelial cells, adhesion experiments with the monocytic U 937 cell line were performed.
IL-1alpha increased ICAM-1,VCAM-1, and E-selectin expression compared to controls. Incubation with IL-1alpha together with linoleic acid reduced the expression of ICAM-1 and VCAM-1 in contrast to palmitic acid. Furthermore, in the presence of linoleic acid a tendency of diminished adhesion of monocytes is seen. The results indicate that a reduced expression of cell adhesion molecules may be relevant to the antiatherogenic effects of linoleic acid. This is in contrast to the properties of palmitic acid.
关于亚油酸，既有有益作用也有促动脉粥样硬化作用的描述。然而，其对人冠状动脉内皮细胞（HCAEC）上细胞黏附分子表达的影响尚未明确。本实验的目的是评估与棕榈酸相比，亚油酸对细胞因子诱导的内皮白细胞黏附分子（细胞间黏附分子 -1，ICAM -1；血管细胞黏附分子 -1，VCAM -1；E -选择素）表达的影响。
HCAEC在微血管内皮细胞生长培养基中培养。在实验中，细胞分别用亚油酸和棕榈酸预孵育（10微摩尔/升，2天）或在对照条件下，之后加入白细胞介素 -1α（IL -1α，测试培养基中10纳克/毫升）1天。使用的单克隆抗体是异硫氰酸荧光素（FITC）标记的抗ICAM -1、FITC标记的抗VCAM -1和FITC标记的抗E -选择素。通过流式细胞荧光术分析表达情况。接下来，为了检测脂肪酸对单核细胞黏附到内皮细胞的影响，用单核细胞U 937细胞系进行黏附实验。
与对照组相比，IL -1α增加了ICAM -1、VCAM -1和E -选择素的表达。与棕榈酸相比，IL -1α与亚油酸一起孵育可降低ICAM -1和VCAM -1的表达。此外，在有亚油酸存在的情况下，可见单核细胞黏附减少的趋势。结果表明细胞黏附分子表达的降低可能与亚油酸的抗动脉粥样硬化作用有关。这与棕榈酸的特性相反。
