Isitor G N, Asgarali Z, Pouching K
Department of Preclinical Sciences, The University of The West Indies, St. Augustine Campus, Trinidad, W.I., Trinidad and Tobago.
Res Vet Sci. 2008 Dec;85(3):418-32. doi: 10.1016/j.rvsc.2008.03.004. Epub 2008 Apr 29.
Nucleated erythrocytes of healthy domestic chicken and ducks, and lymphocytes of healthy Sprague Dawley rats were evaluated for nucleic acid distribution pattern, employing light and fluorescence microscopy procedures, as well as digital imaging analytical methods. The results demonstrate a unique organization of nuclear DNA of mature chicken and duck erythrocytes, as well as immature duck erythrocytes, as delineated spherical nuclear bodies that mostly corresponded with euchromatin zones of the cells in routine Wright-stain blood smears. The nuclear DNA of the rat lymphocytes, on the other hand, was observed as a more diffuse green fluorescing nuclear areas, with punctate variably-sized diffuse areas of RNA red fluorescence. RNA red color fluorescence was also evident in the narrow cytoplasm of the lymphocytes, especially in large lymphocytes, in comparison with the cytoplasm of the mature avian erythrocytes that completely lacked any nucleic acid fluorescence. Nuclear RNA fluorescence was lacking in the mature chicken erythrocytes, compared with those of the mature and immature duck erythrocytes as well as lymphocytes of both avian and rats blood. The significance of these findings lies in the establishment of normal benchmarks for the nuclear and cytoplasmic nucleic acid pattern in eukaryotic cells. These normal benchmarks become valuable in rapid diagnostic situations associated with pathologies, such as the presence of viral nuclear and cytoplasmic inclusion bodies that can alter the nucleic acid pattern of the host cells, and in conditions of cellular abnormal protein aggregations. Variability of cellular nucleic acid pattern can also aid in prognostic assessments of neoplastic conditions.
采用光学显微镜和荧光显微镜检查程序以及数字成像分析方法，对健康家鸡和家鸭的有核红细胞以及健康斯普拉格-道利大鼠的淋巴细胞的核酸分布模式进行了评估。结果表明，成熟鸡和鸭红细胞以及未成熟鸭红细胞的核DNA呈现出独特的组织形式，在常规瑞氏染色血涂片中表现为轮廓清晰的球形核体，大多与细胞的常染色质区域相对应。另一方面，大鼠淋巴细胞的核DNA表现为更弥散的绿色荧光核区域，伴有大小不一的点状弥散RNA红色荧光区域。与完全缺乏任何核酸荧光的成熟禽类红细胞的细胞质相比，淋巴细胞狭窄的细胞质中也有明显的RNA红色荧光，尤其是在大淋巴细胞中。与成熟和未成熟鸭红细胞以及禽类和大鼠血液中的淋巴细胞相比，成熟鸡红细胞缺乏核RNA荧光。这些发现的意义在于确立了真核细胞中核和细胞质核酸模式的正常基准。这些正常基准在与病理学相关的快速诊断情况下具有重要价值，例如存在可改变宿主细胞核和细胞质核酸模式的病毒核和细胞质包涵体的情况，以及细胞异常蛋白质聚集的情况。细胞核酸模式的变异性也有助于肿瘤状况的预后评估。
